Recombinant Rabbit Monoclonal Anti- ROCK1 antibody

Catalog# BRM1127-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthetic peptide of human ROCK1.

Clone ID  5I13

Synonyms P160ROCK, ROCK-I, Rho associated coiled-coil containing protein kinase 1

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry,IHC-P(1:200-1000)

Immunoprecipitation, IP (1:50-100)

Flow Cytometry, Flow-Cyt (1:100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

ROCK1 is a protein serine/threonine kinase also known as rho-associated, coiled-coil-containing protein kinase 1. Other common names are ROKβ and P160ROCK. ROCK1 is a major downstream effector of the small GTPase RhoA and is a regulator of the actomyosin cytoskeleton which promotes contractile force generation. ROCK1 plays a role in cancer and in particular cell motility, metastasis, and angiogenesis. ROCK1 is also the name of the gene that encodes the protein ROCK1, a serine/threonine kinase. ROCK1 is activated when bound to the GTP-bound form of RhoA. The human ROCK1 gene is located on human chromosome 18 with specific location of 18q11.1. The location of the base pair starts at 18,529,703 and ends at 18,691,812 bp and translates into 1354 amino acids.

Reference

1. Rath N, Olson MF (October 2012). "Rho-associated kinases in tumorigenesis: re-considering ROCK inhibition for cancer therapy". EMBO Reports. 13 (10): 900–908.