Recombinant Rabbit Monoclonal Anti- PARP1 antibody

Catalog#  BRM1128-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthetic peptide of human PARP1.

Clone ID  1T3

Synonyms ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; poly(ADP-ribose) polymerase 1; Poly-PARP, PARS

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry,IHC-P (1:200-1000) Immunocytochemistry, ICC/IF (1:200-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. It is the most abundant of the PARP family of enzymes, accounting for 90% of the NAD+ used by the family. PARP1 is mostly present in cell nucleus, but cytosolic fraction of this protein was also reported.

Reference

1. Ha HC, Snyder SH (August 2000). "Poly(ADP-ribose) polymerase-1 in the nervous system". Neurobiology of Disease. 7 (4): 225–39.

2. Xie N, Zhang L, Gao W, Huang C, Zou B (2020). "NAD + metabolism: pathophysiologic mechanisms and therapeutic potential". Signal Transduction and Targeted Therapy. 5 (1): 227.

3. Karpińska, Aneta (2021). "Quantitative analysis of biochemical processes in living cells at a single-molecule level: a case of olaparib–PARP1 (DNA repair protein) interactions" (PDF). Analyst. 146 (23): 7131–7143.