Recombinant Rabbit Monoclonal Anti- Paxillin antibody
Catalog# BRM1130-1
Lot # Check on the product label
Size 50 μl
Isotype IgG
Host Rabbit
Reactivity
Human, mouse
Immunogen A synthetic peptide of human Paxillin.
Clone ID 6Q1
Synonyms Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN
Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Recommend Application
Western Blot, WB (1:1000)
Immunohistochemistry,IHC-P(1:200-1000)
Immunoprecipitation, IP (1:20)
Flow Cytometry, Flow-Cyt (1:100)
Immunocytochemistry, ICC/IF (1:50)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.
Avoid repeated freeze and thaw cycles.
Background
Paxillin is a protein that in humans is encoded by the PXN gene. Paxillin is expressed at focal adhesions of non-striated cells and at costameres of striated muscle cells, and it functions to adhere cells to the extracellular matrix. Mutations in PXN as well as abnormal expression of paxillin protein has been implicated in the progression of various cancers. Human paxillin is 64.5 kDa in molecular weight and 591 amino acids in length. The C-terminal region of paxillin is composed of four tandem double zinc finger LIM domains that are cysteine/histidine-rich with conserved repeats; these serve as binding sites for the protein tyrosine phosphatase-PEST, tubulin and serves as the targeting motif for focal adhesions.
Reference
1."Protein sequence of human PXN (Uniprot ID: P49023)". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). Archived from the original on July 13, 2015. Retrieved July 13, 2015.
2. Shen Y, Schneider G, Cloutier JF, Veillette A, Schaller MD (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". The Journal of Biological Chemistry. 273 (11): 6474–81.
3. Côté JF, Turner CE, Tremblay ML (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST". The Journal of Biological Chemistry. 274 (29): 20550–60.
