Recombinant Rabbit Monoclonal Anti- IKK alpha antibody
Catalog# BRM1133-2
Lot # Check on the product label
Size 100 μl
Isotype IgG
Host Rabbit
Reactivity
Human, mouse, rat
Immunogen A synthetic peptide of human IKK alpha.
Clone ID 7T11
Synonyms CHUK; IKBKA; IKK1; IKKA; NFKBIKA; TCF16; conserved helix-loop-helix ubiquitous kinase; component of inhibitor of nuclear factor kappa B kinase complex; BPS2
Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Recommend Application
Western Blot, WB (1:1000)
Immunohistochemistry, IHC-P (1:50)
Immunoprecipitation, IP (1:50)
Flow Cytometry, Flow-Cyt (1:20)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.
Avoid repeated freeze and thaw cycles.
Background
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK-α) also known as IKK1 or conserved helix-loop-helix ubiquitous kinase (CHUK) is a protein kinase that in humans is encoded by the CHUK gene. The product of the Ph chromosome, the BCR-ABL1 tyrosine kinase activates diverse signaling pathways in leukemic cells from patients with chronic myeloid leukemia (CML) and Ph(+) B-cell acute lymphoblastic leukemia (B-ALL). Coexpression of dominant-negative mutants of IκB kinase α (IKKα)/IKK1 or IKKβ/IKK2 also inhibited lymphoid and myeloid leukemogenesis by BCR-ABL1. Coexpression of dominant-negative mutants of IκB kinase α (IKKα)/IKK1 or IKKβ/IKK2 also inhibited lymphoid and myeloid leukemogenesis by BCR-ABL1. NF-κB is activated through the canonical IKK pathway and plays distinct roles in the pathogenesis of myeloid and lymphoid leukemias induced by BCR-ABL1, validating NF-κB and IKKs as targets for therapy of Ph(+) leukemias.
Reference
1. Mock BA, Connelly MA, McBride OW, Kozak CA, Marcu KB (May 1995). "CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human chromosome 10 and mouse chromosome 19". Genomics 27 (2): 348–51.
2. Hsieh MY, Van Etten RA. IKK-dependent activation of NF-κB contributes to myeloid and lymphoid leukemogenesis by BCR-ABL1. Blood. 2014 Apr 10;123(15):2401-11
