Recombinant Rabbit Monoclonal Anti- Hexokinase II antibody
Catalog# BRM1135-2
Lot # Check on the product label
Size 100 μl
Isotype IgG
Host Rabbit
Reactivity
Human, mouse, rat
Immunogen Recombinant protein of human Hexokinase II.
Clone ID 7H8
Synonyms Hexokinase 2; Hexokinase 2 muscle; Hexokinase type II; Hexokinase-2; HK 2; HK II; HK2; HKII; HxK 2; HxK2; HXK2_HUMAN; Muscle form hexokinase
Content Recombinant protein of human Hexokinase II.
Recommend Application
Western Blot, WB (1:1000)
Immunohistochemistry, IHC-P (1:200-500)
Immunocytochemistry, ICC/IF (1:100)
Immunoprecipitation, IP (1:20-50)
Flow Cytometry, Flow-Cyt (1:50-1:100)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.
Avoid repeated freeze and thaw cycles.
Background
Hexokinase II, also known as Hexokinase B and HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, the first step in most glucose metabolism pathways. Hexokinase II is the predominant hexokinase form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of the HK2 gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009] Hexokinase II is one of four homologous hexokinase isoforms in mammalian cells. The HK2 gene spans approximately 50 kb and consists of 18 exons. There is also an HK2 pseudogene integrated into a long interspersed nuclear repetitive DNA element located on the X chromosome.
Reference
1. Murakami K, Kanno H, Tancabelic J, Fujii H (2002). "Gene expression and biological significance of hexokinase in erythroid cells". Acta Haematologica. 108 (4): 204–9.
2. Okatsu K, Iemura S, Koyano F, Go E, Kimura M, Natsume T, Tanaka K, Matsuda N (Nov 2012). "Mitochondrial hexokinase HKI is a novel substrate of the Parkin ubiquitin ligase". Biochemical and Biophysical Research Communications. 428 (1): 197–202.
3. Schindler A, Foley E (Dec 2013). "Hexokinase 1 blocks apoptotic signals at the mitochondria". Cellular Signalling. 25 (12): 2685–92.
