Recombinant Rabbit Monoclonal Anti- Cyclin T1 antibody
Catalog# BRM1137-1
Lot # Check on the product label
Size 50 μl
Isotype IgG
Host Rabbit
Reactivity
Human, mouse, rat
Immunogen Recombinant protein of human Cyclin T1.
Clone ID 2A7
Synonyms CCNT1; CCNT; CYCT1; HIVE1
Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Recommend Application
Western Blot, WB (1:1000)
Immunohistochemistry, IHC-P (1:100-200)
Immunocytochemistry, ICC/IF (1:200-500)
Immunoprecipitation, IP (1:20-50)
Flow Cytometry, Flow-Cyt (1:100)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.
Avoid repeated freeze and thaw cycles.
Background
Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Positive transcriptional elongation factor b (P-TEFb) contains the catalytic subunit cyclin-dependent kinase 9 (Cdk9) and the regulatory subunit cyclin T. Cyclin T1 and Cdk9 are the key factors of the PTEFb pathways and are overexpressed in the human head and neck carcinoma cell line. Positive expression of cyclin T1 was a good prognostic factor in patients with gastric GISTs. The high expression of cyclin T1 is a favorable prognostic factor in treating patients with stomach adenocarcinoma.
Reference
1. Lin LF, Jin JS, Chen JC, Huang CC, Sheu JH, Chen W, Tsao TY, Hsu CW. Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors. Mol Clin Oncol. 2016 Jun;4(6):971-975.
2. Jin JS, Lin LF, Chen JC, Huang CC, Sheu JH, Chen W, Tsao TY, Hsu CW. Increased cyclin T1 expression as a favorable prognostic factor in treating gastric adenocarcinoma. Oncol Lett. 2015 Dec;10(6):3712-3718. Epub 2015 Sep 25.
