Recombinant Rabbit Monoclonal Anti- CPSF6 antibody

Catalog#  BRM1138-2

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthetic peptide of human CPSF6.

Clone ID  8G1

Synonyms CPSF6; CFIM; CFIM68; HPBRII-4; HPBRII-7; cleavage and polyadenylation specific factor 6; CFIM72

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:2000-20000)

Immunohistochemistry, IHC-P (1:100-200)
Immunocytochemistry, ICC/IF (1:100-200)

Immunoprecipitation, IP (1:10-1:100)

Flow Cytometry, Flow-Cyt (1:10-1:100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Reference

1. Rüegsegger U, Blank D, Keller W (Jan 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Molecular Cell. 1 (2): 243–53.

2. Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SM (Apr 2007). "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". Molecular Biology of the Cell. 18 (4): 1282–92.