Recombinant Rabbit Monoclonal Anti- CDK4 antibody
|
Catalog# |
BRM1139-2 |
|---|---|
|
Lot # |
Check on the product label |
|
Size |
100 μl |
| Isotype |
IgG |
| Host | Rabbit |
|
Reactivity |
Human, mouse, rat |
| Immunogen | Recombinant protein of human Cdk4. |
|
Clone ID |
1C11 |
| Synonyms | CDK4; CMM3; PSK-J3; cyclin-dependent kinase 4; cyclin dependent kinase 4 |
| Content | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA. |
| Recommend Application |
Western Blot, WB (1:1000) The optimal dilutions should be determined by end user. |
| Storage Instruction | Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year. Avoid repeated freeze and thaw cycles. |
|
Background |
Cyclin-dependent kinase 4 also known as cell division protein kinase 4 is an enzyme that in humans is encoded by the CDK4 gene. CDK4 is a member of the cyclin-dependent kinase family. The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16INK4a. This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb).[4] Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. |
|
Reference |
1.Sheppard, K. E.; McArthur, G. A. (2013-10-01). "The Cell-Cycle Regulator CDK4: An Emerging Therapeutic Target in Melanoma". Clinical Cancer Research. 19 (19): 5320–5328. |
