Recombinant Rabbit Monoclonal Anti- BRG1 antibody
Catalog# BRM1140-2
Lot # Check on the product label
Size 100 μl
Isotype IgG
Host Rabbit
Reactivity
Human, mouse, rat
Immunogen A synthetic peptide of mouse BRG1.
Clone ID 3M1
Synonyms SMARCA4; BAF190, BAF190A; BRG1; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; CSS4; SWI/SNF related; matrix associated, actin dependent regulator of chromatin; subfamily a; member 4
Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Recommend Application
Western Blot, WB (1:2000-20000)
Immunohistochemistry, IHC-P (1:100-200)
Immunocytochemistry, ICC/IF (1:200-500)
Immunoprecipitation, IP (1:10-100)
Flow Cytometry, Flow-Cyt (1:100-1:200)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.
Avoid repeated freeze and thaw cycles.
Background
Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Reference
1. "Entrez Gene: SMARCA4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4".
2. DiRenzo J, Shang Y, Phelan M, Sif S, Myers M, Kingston R, Brown M (October 2000). "BRG-1 is recruited to estrogen-responsive promoters and cooperates with factors involved in histone acetylation". Molecular and Cellular Biology. 20 (20): 7541–9.
