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Catalog# |
BRP1181-2 |
|---|---|
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Lot # |
Check on the product label |
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Size |
50 μg |
|
Description |
Recombinant human IL-10 is produced by the mammalian system, with the target gene encoding Ser19-Asn178 expressed. |
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Synonyms |
IL10, CSIF, GVHDS, IL-10, IL10A, TGIF, interleukin 10 |
|
Source |
Human Cells |
|
Uniprot No. |
P22301 |
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Purity |
>95% by SDS-PAGE |
|
Endotoxin level |
<0.01 EU/µg by LAL test |
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Formulation |
Lyophilized from a 0.2 μm filtered solution of PBS, 4%Mannitol, pH 7.4. |
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Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
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Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
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Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
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Background |
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and TYK2 respectively. |
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Reference |
1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence". Immunogenetics. 46 (2): 120–128. 2. Mosser DM, Zhang X (December 2008). "Interleukin-10: new perspectives on an old cytokine". Immunological Reviews. 226 (1): 205–218. |
