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Catalog# |
BRP1183-1 |
|---|---|
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Lot # |
Check on the product label |
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Size |
10 μg |
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Description |
Recombinant human IL-11 is produced by the yeast system, with the target gene encoding Gly23-Leu199 expressed. |
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Synonyms |
IL11, AGIF, IL-11, interleukin 11 |
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Source |
P.Pichia |
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Uniprot No. |
P20809 |
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Purity |
>95% by SDS-PAGE |
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Endotoxin level |
<0.01 EU/µg by LAL test |
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Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 2% Glycine, pH 7.2. |
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Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
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Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
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Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
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Background |
Interleukin 11 (IL-11 or adipogenesis inhibitory factor) is a protein that in humans is encoded by the IL11 gene. IL-11 is a cytokine that was first isolated in 1990 from bone marrow-derived fibrocyte-like stromal cells. It was initially thought to be important for hematopoiesis, notably for megakaryocyte maturation, but subsequently shown to be redundant for platelets, and for other blood cell types, in both mice and humans. It was developed as a recombinant protein (rhIL-11) as the drug substance oprelvekin. |
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Reference |
1. Kawashima I, Ohsumi J, Mita-Honjo K, Shimoda-Takano K, Ishikawa H, Sakakibara S, et al. (June 1991). "Molecular cloning of cDNA encoding adipogenesis inhibitory factor and identity with interleukin-11". FEBS Letters. 283 (2): 199–202. 2. McKinley D, Wu Q, Yang-Feng T, Yang YC (July 1992). "Genomic sequence and chromosomal location of human interleukin-11 gene (IL11)". Genomics. 13 (3): 814–9. 3. Paul SR, Bennett F, Calvetti JA, Kelleher K, Wood CR, O'Hara RM, et al. (October 1990). "Molecular cloning of a cDNA encoding interleukin 11, a stromal cell-derived lymphopoietic and hematopoietic cytokine". Proceedings of the National Academy of Sciences of the United States of America. 87 (19): 7512–6. 4. Nandurkar HH, Robb L, Tarlinton D, Barnett L, Köntgen F, Begley CG (September 1997). "Adult mice with targeted mutation of the interleukin-11 receptor (IL11Ra) display normal hematopoiesis". Blood. 90 (6): 2148–59. |
