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Catalog# |
BRP1184-2 |
|---|---|
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Lot # |
Check on the product label |
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Size |
50 μg |
|
Description |
Recombinant human IL-12 is produced by the mammalian system, with the target gene encoding Arg23-Ser219& Ile23-Ser328 expressed. |
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Synonyms |
Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, NK cell, IL12A , NKSF1 stimulatory factor chain 1 |
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Source |
Human Cells |
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Uniprot No. |
P29459&P29460 |
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Purity |
>95% by SDS-PAGE |
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Endotoxin level |
<0.01 EU/µg by LAL test |
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Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
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Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
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Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
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Background |
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, helper T cells and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12. IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35. Despite sharing many structural features and molecular partners, they mediate surprisingly diverse functional effects. |
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Reference |
1. Kaliński P, Hilkens CM, Snijders A, Snijdewint FG, Kapsenberg ML (July 1997). "IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells". Journal of Immunology. 159 (1): 28–35. 2. Vignali DA, Kuchroo VK (July 2012). "IL-12 family cytokines: immunological playmakers". Nature Immunology. 13 (8): 722–8. |
