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Catalog# |
BRP1189-1 |
|---|---|
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Lot # |
Check on the product label |
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Size |
10 μg |
|
Description |
Recombinant human IL-18 is produced by the E.coli system, with the target gene encoding Tyr37-Asp193 expressed. |
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Synonyms |
IL18, IGIF, IL-18, IL-1g, IL1F4, interleukin 18 |
|
Source |
E.coli |
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Uniprot No. |
Q14116 |
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Purity |
>95% by SDS-PAGE |
|
Endotoxin level |
<0.01 EU/µg by LAL test |
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Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Sucrose, 0.1% Tween80,pH 7.4. |
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Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
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Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
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Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
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Background |
Interleukin-18 (IL-18), also known as interferon-gamma inducing factor is a protein which in humans is encoded by the IL18 gene.[ The protein encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, and liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases. |
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Reference |
1. Okamura H, Tsutsi H, Komatsu T, Yutsudo M, Hakura A, Tanimoto T, et al. (November 1995). "Cloning of a new cytokine that induces IFN-gamma production by T cells". Nature. 378 (6552): 88–91. 2. Nolan KF, Greaves DR, Waldmann H (July 1998). "The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci". Genomics. 51 (1): 161–163. 3. Nakamura K, Okamura H, Wada M, Nagata K, Tamura T (February 1989). "Endotoxin-induced serum factor that stimulates gamma interferon production". Infection and Immunity. 57 (2): 590–595. |
