Recombinant Human IL-18 Protein

Check on the product label

50 μg

Recombinant human IL-18 is produced by the E.coli system, with the target gene encoding Tyr37-Asp193 expressed.

IL18, IGIF, IL-18, IL-1g, IL1F4, interleukin 18

E.coli

Q14116

>95% by SDS-PAGE

<0.01 EU/µg by LAL test

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Sucrose, 0.1% Tween80,pH 7.4.

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.)

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Interleukin-18 (IL-18), also known as interferon-gamma inducing factor is a protein which in humans is encoded by the IL18 gene.[ The protein encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, and liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases.

1. Okamura H, Tsutsi H, Komatsu T, Yutsudo M, Hakura A, Tanimoto T, et al. (November 1995). "Cloning of a new cytokine that induces IFN-gamma production by T cells". Nature. 378 (6552): 88–91.

2. Nolan KF, Greaves DR, Waldmann H (July 1998). "The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci". Genomics. 51 (1): 161–163.

3. Nakamura K, Okamura H, Wada M, Nagata K, Tamura T (February 1989). "Endotoxin-induced serum factor that stimulates gamma interferon production". Infection and Immunity. 57 (2): 590–595.