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Catalog# |
BRP1192-1 |
|---|---|
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Lot # |
Check on the product label |
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Size |
10 μg |
|
Description |
Recombinant human IL-1b is produced by the E.coli system, with the target gene encoding Ala117-Ser269 expressed. |
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Synonyms |
IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta |
|
Source |
E.coli |
|
Uniprot No. |
P01584 |
|
Purity |
>95% by SDS-PAGE |
|
Endotoxin level |
<0.01 EU/µg by LAL test |
|
Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 8% Trehalose, 4% Mannitol, 0.02% Tween-80, pH 7.4. |
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Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
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Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
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Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
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Background |
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. |
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Reference |
1. Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA (December 1984). "Nucleotide sequence of human monocyte interleukin 1 precursor cDNA". Proceedings of the National Academy of Sciences of the United States of America. 81 (24): 7907–7911. 2. "Catabolin" is the name given by Jeremy Saklatvala for IL-1 alpha. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, et al. (1985). "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs". Nature. 315 (6021): 641–647. 3. Clark BD, Collins KL, Gandy MS, Webb AC, Auron PE (October 1986). "Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene". Nucleic Acids Research. 14 (20): 7897–7914. 4. Bensi G, Raugei G, Palla E, Carinci V, Tornese Buonamassa D, Melli M (1987). "Human interleukin-1 beta gene". Gene. 52 (1): 95–101. |
