|
Catalog# |
BRP1201-1 |
|---|---|
|
Lot # |
Check on the product label |
|
Size |
10 μg |
|
Description |
Recombinant human G-CSF is produced by the E.coli system, with the target gene encoding Thr31-Pro204 expressed. |
|
Synonyms |
CSF3, C17orf33, CSF3OS, GCSF, colony stimulating factor 3 |
|
Source |
E.coli |
|
Uniprot No. |
P09919-2 |
|
Purity |
>95% by SDS-PAGE |
|
Endotoxin level |
<0.01 EU/µg by LAL test |
|
Formulation |
Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0. |
|
Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
|
Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
|
Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
|
Background |
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. |
|
Reference |
1. Deotare U, Al-Dawsari G, Couban S, Lipton JH (September 2015). "G-CSF-primed bone marrow as a source of stem cells for allografting: revisiting the concept". Bone Marrow Transplantation. 50 (9): 1150–1156. 2. Tay J, Levesque JP, Winkler IG (February 2017). "Cellular players of hematopoietic stem cell mobilization in the bone marrow niche". International Journal of Hematology. 105 (2): 129–140. |
