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Catalog# |
BRP1204-1 |
|---|---|
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Lot # |
Check on the product label |
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Size |
10 μg |
|
Description |
Recombinant human FGF-1 is produced by the E.coli system, with the target gene encoding Phe16-Asp155 expressed. |
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Synonyms |
FGF1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1, fibroblast growth factor 1 |
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Source |
E.coli |
|
Uniprot No. |
P05230 |
|
Purity |
>95% by SDS-PAGE |
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Endotoxin level |
<1 EU/µg by LAL test |
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Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Mops, 50mM Na2SO4, 1mM EDTA , pH 6.7. |
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Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
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Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
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Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
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Background |
Fibroblast growth factor 1 (FGF-1) also known as acidic fibroblast growth factor (aFGF), is a growth factor and signaling protein encoded by the FGF1 gene. It is synthesized as a 155 amino acid polypeptide, whose mature form is a non-glycosylated 17-18 kDa protein. Fibroblast growth factor protein was first purified in 1975, but soon afterwards others using different conditions isolated acidic FGF, Heparin-binding growth factor-1, and Endothelial cell growth factor-1. Gene sequencing revealed that this group was actually the same growth factor and that FGF1 was a member of a family of FGF proteins. |
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Reference |
1. Dionne CA, Crumley G, Bellot F, Kaplow JM, Searfoss G, Ruta M, Burgess WH, Jaye M, Schlessinger J (September 1990). "Cloning and expression of two distinct high-affinity receptors cross-reacting with acidic and basic fibroblast growth factors". The EMBO Journal. 9 (9): 2685–92. 2. Jaye M, Howk R, Burgess W, Ricca GA, Chiu IM, Ravera MW, O'Brien SJ, Modi WS, Maciag T, Drohan WN (August 1986). "Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization". Science. 233 (4763): 541–5. 3. Burgess WH, Maciag T (1989). "The heparin-binding (fibroblast) growth factor family of proteins". Annual Review of Biochemistry. 58: 575–606. |
