Human Emmprin/sCD147 ELISA Kit

Catalog No.: BEK1047

Size: 96T

Range: 31.2 pg/ml-2000 pg/ml

Sensitivity < 2pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months. 

Application: For quantitative detection of Emmprin / soluble CD147 in tissue lysates or cell culture supernates. The significance of detection in human

serum, plasma, body fluids is undetermined.

Introduction

Cluster of Differentiation 147 (CD147) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or Basigin (BSG), is a member of the immunoglobulin superfamily, It interacts with fibroblasts and stimulates expression of MMPs, and plays an important role in tumor invasiveness and metastasis. This protein is a determinant for the Ok blood group system, and it has been shown to be an essential receptor on red blood cells for the human malaria parasite, Plasmodium falciparum.

Principle of the Assay  

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human- Emmprin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Emmprin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Emmprin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Emmprin can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-Human Emmprin antibody
2. Lyophilized Human Emmprin standards: 2 tubes (10ng / tube)                     
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Human Emmprin antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer(25X): 30ml

Note: Reconstitute standards and test samples with Kit Component 3.

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1L