Monoclonal Anti- Bone morphogenetic protein receptor type II, BMPR2

Catalog#  BMA1047

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Mouse

Reactivity

Human, mouse, rat

Product Form Liquid

Purification Protein A purified

Immunogen 

Recombinant protein

Recommend Application

Western Blot, WB (1:200-1:500)

Immunohistochemistry,IHC-P (1:20-1:100)

Immunocytochemistry, ICC (1:50-200)

Other applications have not been tested.

 The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Bone morphogenetic protein receptor type II, BMPR2 is a serine/threonine receptor kinase. It binds bone morphogenetic proteins, members of the TGF beta superfamily of ligands. BMPs are involved in a host of cellular functions including osteogenesis, cell growth and cell differentiation. Unlike the TGFβtype II receptor, which has a high affinity for TGF-β1, BMPR2 does not have a high affinity for BMP-2, BMP-7 and BMP-4, unless it is co-expressed with a type I BMP receptor.1 The low affinity for ligands suggests that BMPR2 may differ in the from other type II TGF beta receptors in that the ligand may bind the type I receptor first.2

Reference

1.       Gilboa, L; Nohe A, Geissendorfer T, Sebald W, Henis YI, Knaus P. (March 2000). "Bone morphogenetic protein receptor complexes on the surface of live cells: a new oligomerization mode for serine/threonine kinase receptors". Mol Biol Cell. 11 (3).

2.       Kirsch, T; Nickel J, Sebald W. (July 2000). "BMP-2 antagonists emerge from alterations in the low-affinity binding epitope for receptor BMPR-II". EMBO J. 19 (13): 3314.