Monoclonal Anti- E-Cadherin (CDH1)

Catalog#  BMA1048

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Rabbit

Reactivity

Human

Product Form Liquid

Purification Protein A affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the region of human E-cadherin.

Recommend Application

Western Blot, WB (1:200-1:500)

Immunohistochemistry, IHC-P(1:20-1:100)

Immunocytochemistry,ICC/IF (1:20-1:100)

Flow Cytometry, Flow-Cyt (1:20-1:50)

 Other applications have not been tested.

 The optimal dilutions should be determined by end user.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Cadherin-1 also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene.1 E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. It has 16 exons spanning approximately 100 kb of genomic DNA. The gene structure is similar to that of other cadherins.2 E-cadherin plays a central part in the process of epithelial morphogenesis. Expression of this protein is downregulated during the acquisition of metastatic potential at late stages of epithelial tumour progression.3

Reference

1.       Huntsman DG, Caldas C (Mar 1999). "Assignment1 of the E-cadherin gene (CDH1) to chromosome 16q22.1 by radiation hybrid mapping". Cytogenet Cell Genet 83 (1-2): 82–3.

2.       Berx, G., Staes, K., van Hengel, J., Molemans, F., Bussemakers, M. J. G., van Bokhoven, A., van Roy, F. Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1). Genomics 26: 281-289, 1995.

3.       Batlle, E., Sancho, E., Franci, C., Dominguez, D., Monfar, M., Baulida, J., Garcia de Herreros, A. The transition factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nature Cell Biol. 2: 84-89, 2000.