Monoclonal Anti- Caldesmon

Catalog#  BMA1049

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Rabbit

Reactivity

Human, mouse, rat

Product Form Liquid

Purification Protein A affinity purified

Immunogen 

A synthetic peptide within C-terminal human Caldesmon.

Recommend Application

Western Blot, WB (1:500-1:1,000)

Immunohistochemistry, IHC-P (1:20-1:100)

Immunocytochemistry, ICC/IF (1:20-1:100)

Flow Cytometry, Flow-Cyt (1:20-1:50)

Other applications have not been tested.

 The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Caldesmon is a protein that in humans is encoded by the CALD1 gene. Caldesmon (CDM) is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. Domain mapping and physical studies suggest that CDM is an elongated molecule with an N-terminal myosin/calmodulin-binding domain and a C-terminal tropomyosin/actin/calmodulin-binding domain separated by a 40-nm-long central helix.1 The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells.2 The conserved domain of this protein possesses the binding activities to Ca++-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca++-dependent inhibition of smooth muscle contraction. 

Reference

1.       Humphrey, M. B., Herrera-Sosa, H., Gonzalez, G., Lee, R., Bryan, J. Cloning of cDNAs encoding human caldesmons. Gene 112: 197-204, 1992.

2.       Hayashi, K., Yano, H., Hashida, T., Takeuchi, R., Takeda, O., Asada, K., Takahashi, E., Kato, I., Sobue, K. Genomic structure of the human caldesmon gene. Proc. Nat. Acad. Sci. 89: 12122-12126, 1992.