Monoclonal Anti- Neuropilin 1

Catalog#  BMA1050

Lot # Check on the product label

Size 100μg

Isotype IgG

Host Rabbit

Reactiv ity

Human, mouse, rat

Product Form Liquid

Purification Protein A affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the N-terminal of Neuropilin 1.

Recommend Application

Western Blot, WB (1:500-1:2,000)

Immunohistochemistry, IHC-P(1:20-1:100)

Immunocytochemistry, ICC/IF(1:20-1:100)

Flow Cytometry, Flow-Cyt (1:20-1:50)

 Other applications have not been tested.

 The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction  

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Neuropilin-1 (NRP-1) is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor and semaphorin family members.[1] The NRP-1 gene contains 17 exons and spans 120 kb,[2] maps to 10p12. [3]NRP-1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

Reference

1.       Soker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M (April 1998). "Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor". Cell 92 (6): 735–45.

2.       Rossignol, M., Gagnon, M. L., Klagsbrun, M. Genomic organization of human neuropilin-1 and neuropilin-2 genes: identification and distribution of splice variants and soluble isoforms. Genomics 70: 211-222, 2000.

3.       Rossignol, M., Beggs, A. H., Pierce, E. A., Klagsbrun, M. Human neuropilin-1 and neuropilin-2 map to 10p12 and 2q34, respectively. Genomics 57: 459-460, 1999.