Monkey C-Reactive Protein, CRP ELISA Kit

Catalog No.: BZEK2861

Size: 96T

Range: 200 μg/L - 3200 μg/L

Sensitivity: 10 μg/L

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of CRP in monkey serum, plasma, tissue homogenate or cell culture supernatant.

Introduction

C-reactive protein (CRP) is an annular (ring-shaped) pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. CRP is synthesized by the liver in response to factors released by macrophages, T cells and fat cells (adipocytes). It is a member of the pentraxin family of proteins.[6] It is not related to C-peptide (insulin) or protein C (blood coagulation). C-reactive protein was the first pattern recognition receptor (PRR) to be identified.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- CRP antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- CRP antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.

Kit components

1. One 96-well plate pre-coated with anti- monkey CRP antibody

2. Standard: 0.5 ml (6400 μg/L)

3. Standard diluent buffer: 1.5 ml

4. Wash buffer (30×): 20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti- monkey CRP antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A: 6 ml

9. TMB substrate B: 6 ml

10. Plate sealer: 2

11. Hermetic bag: 1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L