Monkey Cortisol ELISA Kit

Catalog No.: BZEK2869

Size: 96T

Range: 12.5 μg/L - 200 μg/L

Sensitivity: 1 μg/L

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of Cortisol in monkey serum, plasma, tissue homogenate or cell culture supernatant.

Introduction

Cortisol is a steroid hormone, in the glucocorticoid class of hormones and a stress hormone. It is produced in many animals, mainly by the zona fasciculata of the adrenal cortex in the adrenal gland. It is produced in other tissues in lower quantities. It is released with a diurnal cycle and its release is increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation. Many of these functions are carried out by cortisol binding to glucocorticoid or mineralocorticoid receptors inside the cell, which then bind to DNA to impact gene expression.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- Cortisol antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- Cortisol antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cortisol amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cortisol can be calculated.

Kit components

1. One 96-well plate pre-coated with anti- monkey Cortisol antibody

2. Standard: 0.5ml (400 μg/L)

3. Standard diluent buffer: 1.5 ml

4. Wash buffer (30×): 20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti- monkey Cortisol antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A:  6ml

9. TMB substrate B:  6ml

10. Plate sealer:   2

11. Hermetic bag:  1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L