Monkey Apolipoprotein C3, APOC3 ELISA Kit

Catalog No.: BZEK2875

Size: 96T

Range: 0.5 μg/ml - 8 μg/ml

Sensitivity: 0.1 μg/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of APOC3 in monkey serum, plasma, tissue homogenate or cell culture supernatant.

Introduction

Apolipoprotein C-III also known as apo-CIII, and apolipoprotein C3, is a protein that in humans is encoded by the APOC3 gene. Apo-CIII is secreted by the liver as well as the small intestine, and is found on triglyceride-rich lipoproteins such as chylomicrons, very low density lipoprotein (VLDL), and remnant cholesterol. ApoC-III is a relatively small protein containing 79 amino acids that can be glycosylated at threonine-74. The most abundant glycoforms are characterized by an O-linked disaccharide galactose linked to N-acetylgalactosamine (Gal–GalNAc), further modified with up to two sialic acid residues. Less abundant glycoforms are characterized by more complex and fucosylated glycan moieties.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- APOC3 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- APOC3 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the APOC3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of APOC3 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti- monkey APOC3 antibody

2. Standard: 0.5ml (16 μg/ml)

3. Standard diluent buffer: 1.5 ml

4. Wash buffer (30×): 20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti- monkey APOC3 antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A:  6ml

9. TMB substrate B:  6ml

10. Plate sealer:   2

11. Hermetic bag:  1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L