Mouse BCMA / TNFRSF17 ELISA Kit

Catalog No.: BEK1339

Size: 96T

Range: 31.2 pg/ml - 2000 pg/ml

Sensitivity < 7 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months. 

Application: For quantitative detection of BCMA / TNFRSF17 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Introduction

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF). Serum B-cell maturation antigen (sBCMA) is the cleaved form of BCMA, found at low levels in the serum of normal patients and generally elevated in patients with multiple myeloma (MM).

Principle of the Assay  

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BCMA / TNFRSF17 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BCMA / TNFRSF17 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BCMA / TNFRSF17 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BCMA / TNFRSF17 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-mouse BCMA / TNFRSF17 antibody
2. Lyophilized mouse BCMA / TNFRSF17 standards: 2 tubes (10 ng / tube)                     
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-mouse BCMA / TNFRSF17 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
11. Note: Reconstitute standards and test samples with Kit Component 3.

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1L