Mouse MCP-1 ELISA Kit
Size: 96T
Range: 15.6 pg/ml-1000 pg/ml
Sensitivity < 6 pg/ml
Application: For quantitative detection of MCP-1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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Price: $350.00
Catalog No.: BEK1143
Size: 96T
Range: 15.6 pg/ml-1000 pg/ml
Sensitivity < 6 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of MCP-1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction
Monocyte chemotactic protein-1(MCP-1), known as chemmkine(C-C motif) ligand 2(CCL2) or small inducible cytokine A2, is a small cytokine belonging to the CC chemokine family. The CCL2 gene is located on chromosome 17 (17q11.2-q21.1) in humans, and spans 1,927 bases. It has three exons and two introns, and produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. The predicted weight is 11.025 kiloDaltons (kDa). MCP-1 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It induces amylin expression through ERK1/ ERK2/ JNK-AP1 and NF-κB related signaling pathways independent of CCR2.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MCP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MCP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-1 can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Mouse MCP-1 antibody
- Lyophilized Mouse MCP-1 standards: 2 tubes (10 ng/ tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-Mouse MCP-1 antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L