Mouse MIP-2 ELISA Kit
Size: 96T
Range: 15.6 pg/ml-1000 pg/ml
Sensitivity < 5 pg/ml
Application: For quantitative detection of MIP-2 in mouse serum, body fluids, tissue lysates or cell culture supernatants.
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Price: $380.00
Catalog No.: BEK1153
Size: 96T
Range: 15.6 pg/ml-1000 pg/ml
Sensitivity < 5 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of MIP-2 in mouse serum, body fluids, tissue lysates or cell culture supernatants.
Introduction
The major intrinsic protein of the ocular lens fiber membrane (MIP) is an abundant protein that appears during differentiation of the ocular lens and has a molecular weight of about 26,000 daltons. The MIP gene is 3.6 kb, contains 4 exons separated by introns ranging in size from 0.4 to 1.6 kb, and is present in single copy in the haploid human genome.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MIP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MIP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MIP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIP-2 can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Mouse MIP-2 antibody
- Lyophilized Mouse MIP-2 standards: 2 tubes (10 ng / tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-Mouse MIP-2 antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L