Human MIP-1α ELISA Kit
Size: 96T
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity < 10 pg/ml
Application: For quantitative detection of MIP-1α in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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Price: $350.00
Catalog No.: BEK1152
Size: 96T
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity < 10 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of MIP-1α in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction
Macrophage inflammatory protein-1(MIP-1a), also known as Chemokine (C-C motif) ligand 3(CCL3) or LD78, is a cytokine belonging to the CC chemokine family. The LD78 gene chromosome 17q21.1-q21.3. It is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Sherry et al. (1988) demonstrated 2 protein components of MIP1, called by them alpha and beta. MIP-1a is an important mediator of virus-induced inflammation in vivo, and also an important second signal for mast cell degranulation in the conjunctiva and for acute-phase disease, possibly through interaction with CCR1, its chemokine receptor.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MIP-1α polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MIP-1α polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MIP-1α amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIP-1α can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Human MIP-1α antibody
- Lyophilized Human MIP-1α standards: 2 tubes (10ng / tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-Human MIP-1α antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer(25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L