Human MMP-3 ELISA Kit
Size: 96T
Range: 156 pg/ml-10,000 pg/ml
Sensitivity < 10 pg/ml
Application: For quantitative detection of MMP-3 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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Price: $350.00
Catalog No.: BEK1159
Size: 96T
Range: 156 pg/ml-10,000 pg/ml
Sensitivity < 10 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of MMP-3 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction
Matrix metalloproteinase-3 (MMP-3) also known as Stromelysin-1 is an enzyme that in humans is encoded by the MMP3 gene. This gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. It is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-3, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-3 can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Human MMP-3 antibody
- Lyophilized Human MMP-3 standards: 2 tubes (10 ng / tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-human MMP-3 antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer(25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L