Human MMP-7 ELISA Kit

Catalog No.: BEK1160

Size: 96T

Range: 156 pg/ml-10,000 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of MMP-7 in human serum , plasma, urine, cell culture supernatant or tissue samples.

Introduction

Matrix metalloproteinase-7 (MMP-7) also known as Matrilysin is an enzyme that in humans is encoded by the MMP7 gene, which maps to 11q21-q22. It is a member of the MMP family, these proteins are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This protein has 267 amino acids and is significantly shorter than stromelysin or collagenase, It was expressed in basal epithelial cells during the migration proliferation phase of corneal wound healing after excimer keratectomy. Crawford et al. (2002) hypothesized that MMP7 expression in preneoplastic pancreatic lesions applies apoptotic selective pressure to susceptible epithelial cells, resulting in selective expansion of a duct-like epithelium resistant to apoptotic stimuli.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MMP-7 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MMP-7 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-7 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-MMP-7 antibody

2. Standard: 0.5ml (13500pg /mL)

3. Standard diluent buffer: 1.5 ml

4. Wash buffer (30×): 20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti-MMP-7 antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A:  6ml

9. TMB substrate B:  6ml

10. Plate sealer:   2

11. Hermetic bag:  1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L