Polyclonal Anti- Vang-like protein 1, VANGL1 Antibody

Catalog#  BPA1108

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Rabbit

Reactivity Human

Product Form Liquid

Purification Peptide affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the region of human VANGL1.

Recommend Application

Western Blot, WB (1:100-200)

Immunocytochemistry, ICC (1:50-1:100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Vang-like protein 1 (VANGL1), also called Loop-tail protein 2 homolog (LPP2), is encoded by the VANGL1 gene, This gene contains 8 exons and it maps to chromosome 1p13. The VANGL1 protein is a 524-amino acid , 65KD protein, which has 4 transmembrane domains and a C-terminal ser/thr-x-val motif. Yagyu et al. (2002) found that downregulation of VANGL1 expression suppressed growth of a human hepatoma cell line. Kalabis et al. (2006) found that VANGL1 overexpression enhanced this migration and wound closure in cultured cells.

Reference

1. Yagyu, R., Hamamoto, R., Furukawa, Y., Okabe, H., Yamamura, T., Nakamura, Y. Isolation and characterization of a novel human gene, VANGL1, as a therapeutic target for hepatocellular carcinoma. Int. J. Oncol. 20: 1173-1178, 2002.

2. Katoh, M. Molecular cloning and characterization of Strabismus 2 (STB2). Int. J. Oncol. 20: 993-998, 2002.

3. Kalabis, J., Rosenberg, I., Podolsky, D. K. Vangl1 protein acts as a downstream effector of intestinal trefoil factor (ITF)/TFF3 signaling and regulates wound healing of intestinal epithelium. J. Biol. Chem. 281: 6434-6441, 2006.