Monoclonal Anti- Synaptophysin Antibody

Catalog# BMA1038

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # S7

Host Mouse

Reactivity Human, mouse, rat

Product Form Liquid

Purification Peptide affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the TOPO_MOD residues of mouse Synaptophysin.

Recommend Application

Western Blot, WB (1:100-1:500)

Immunohistochemistry, IHC-P(1:20-1:100)

Immunocytochemistry, ICC (1:20-1:100)

Flow Cytometry, Flow-Cyt (1:20-1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Synaptophysin, also known as the major synaptic vesicle protein p38, is an integral membrane protein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass, accounting for about 10% of total vesicle protein. The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.

Reference

1. Kwon, S. E., Chapman, E. R. Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 70: 847-854, 2011.

2. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996). "Comparative evaluation of synaptophysin-based methods for quantification of synapses". J. Neurocytol. 25 (12): 821–8.