Monoclonal Anti- Terminal deoxynucleotidyl transferase, TDT Antibody

Catalog# BMA1039  

Lot # Check on the product label

Size 100 μg

Isotype IgG2b

Clone # N153

Host Mouse

Reactivity Human, mouse

Product Form Liquid

Purification Peptide affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of human TDT.

Recommend Application

Western Blot,WB (1:1000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a DNA polymerase expressed in immature lymphocytes of the thymus and bone marrow, as well as certain leukemic cells. TDT cDNA contains an open reading frame of 1530 base pairs (bp) corresponding to a protein containing 510 amino acids. TDT is a useful marker in the diagnosis of acute lymphoblastic leukemia (ALL) (French-American-British [FAB] L1 and L2) and is most useful in distinguishing ALL from mature B-lymphoid neoplasms, such as Burkitt lymphoma (FAB L3) and other lymphoid malignancies.

Reference

1. Yang-Feng, T. L., Landau, N. R., Baltimore, D., Francke, U. The terminal deoxynucleotidyltransferase gene is located on human chromosome 10 (10q23-q24) and on mouse chromosome 19. Cytogenet. Cell Genet. 43: 121-126, 1986.

2. Riley, L. K., Morrow, J. K., Danton, M. J., Coleman, M. S. Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5-prime flanking region. Proc. Nat. Acad. Sci. 85: 2489-2493, 1988.

3. Faber J, Kantarjian H, Roberts MW, Keating M, Freireich E, Albitar M (January 2000). "Terminal deoxynucleotidyl transferase-negative acute lymphoblastic leukemia". Arch. Pathol. Lab. Med. 124 (1): 92–7.