Ferritin (H chain) Protein (Active)

Catalog# BRP1015

Lot # Check on the product label

Size 1 mg

Description

Recombinant full length human ferritin (H chain) protein with molecular weight 21.7 kDa.

Synonyms Ferritin heavy chain, Cell proliferation-inducing gene 15 protein, FTH, FTH1, FTHL6

Source E. Coli

Purity >95% by SDS-PAGE

Formulation Liquid

Concentration 2 mg/ml

Application

ELISA

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

Each vial contains 10 mM Tris-HCL (pH8.8 ± 0.2) with 0.05% sodium azide.

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. It is a protein of 450 kDa consisting of 24 subunits that is present in every cell type. In vertebrates, these subunits are both the light (L) and the heavy (H) type with an apparent molecular weight of 19 kDA or 21 kDA respectively; their sequences are about 50% homologous. Ferritin heavy chain (FHC), the primary iron storage factor is an essential mediator of the antioxidant and protective activities of NF-kappa B. FHC is induced downstream of NF-kappa B and is required to prevent sustained JNK activation and, thereby, apoptosis triggered by TNF alpha. FHC-mediated inhibition of JNK signaling depends on suppressing ROS accumulation and is achieved through iron sequestration. The results suggest modulation of FHC or, more broadly, of iron metabolism as a potential approach for anti-inflammatory therapy.

Reference

1. Theil, E. (1987). "Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms.". Annual review of biochemistry 56 (1): 289–315.

2. Pham, C. G., Bubici, C., Zazzeroni, F., Papa, S., Jones, J., Alvarez, K., Jayawardena, S., De Smaele, E., Cong, R., Beaumont, C., Torti, F. M., Torti, S. V., Franzoso, G. Ferritin heavy chain upregulation by NF-kappa-B inhibits TNF-alpha-induced apoptosis by suppressing reactive oxygen species. Cell 119: 529-542, 2004.