FGF basic / FGF2 Protein (Active)

Catalog# BRP1019

Lot # Check on the product label

Size 100 µg

Description

Recombinant human basic FGF / FGF2 protein with molecular weight 18 kDa.

Synonyms Fibroblast growth factor 2, FGF2, Basic fibroblast growth factor, BFGF, FGFB, Heparin-binding growth factor 2

Source E. Coli

Purity >95% by HPLC & SDS-PAGE

Formulation Lyophilized

Reconstitution

Reconstitute with sterile 18MΩ-cm H₂O.

Endotoxin level < 0.1 ng/μg (1 EU/μg) by LAL test

Maximal absorption wave 277 ± 3 nm

Dimmers < 1% by SDS-PAGE

Biological Activity 

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is < 2 ng/ml corresponding to a specific activity of 1.7×10⁶IU/mg.

Storage buffer

Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.

Storage & Expiration

Ship at 4℃. Upon receipt, store at -20℃ or -80℃ for long term. After reconstituting, it can store at 4℃ for a week, it is recommended to add 0.1% HSA or BSA for long term.

Avoid repeated freeze and thaw cycles.

Background 

Fibroblast growth factor, basic (FGFB) also know as Fibroblast growth factor 2 (FGF2) is a wide-spectrum mitogenic, angiogenic, and neurotrophic factor that is expressed at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary. FGF-2 participates in controlling fates, migration and differentiation of neuronal cells, whereas it is not essential for their proliferation. FGF-2 accelerates fracture healing and prevents nonunion in primates, and therefore propose that it is a potent bone anabolic agent for clinical use. It could also be used in vivo to facilitate the mobilization and differentiation of resident cardiac precursors in the treatment of cardiac diseases.

Reference

1. Dono, R., Texido, G., Dussel, R., Ehmke, H., Zeller, R. Impaired cerebral cortex development and blood pressure regulation in FGF2-deficient mice. EMBO J. 17: 4213-4225, 1998.

2. Kawaguchi, H., Nakamura, K., Tabata, Y., Ikada, Y., Aoyama, I., Anzai, J., Nakamura, T., Hiyama, Y., Tamura, M. Acceleration of fracture healing in nonhuman primates by fibroblast growth factor-2. J. Clin. Endocr. Metab. 86: 875-880, 2001.

3. Rosenblatt-Velin, N., Lepore, M. G., Cartoni, C., Beermann, F., Pedrazzini, T. FGF-2 controls the differentiation of resident cardiac precursors into functional cardiomyocytes. J. Clin. Invest. 115: 1724-1733, 2005.