AEC Horseradish Peroxidase Chromogenic Kit (Red)
Size: 100 ml
Lot # Check on the product label
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Price: $45.00
Catalog# BWR1066
Size: 100 ml
Lot # Check on the product label
Introduction
1. AEC is a widely used chromogen for immunohistochemical staining. AEC produces a red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media.
2. 3 amino9 ethyl carbazole (AEC) is one of the important Chromogenic reagent of peroxidase with the color of bright red. It can be used for immunohistochemistry and spot detection. The disadvantage of AEC is that it is soluble in organic solvents and must be sealed by water-soluble sealants.
Kit Components
|
Components |
Size |
|
Chromogenic Reagent A (AEC Concentrated Solution , 20×) |
3 ml |
|
Chromogenic Reagent B (Acetate Concentrated Buffer (containing H2O2, 20×) |
3 ml |
Storage
Store at 4℃ for frequence use, or store at -20℃ for long term.
Protocol
1. Wash with TBS or PBS for 4 times, 5 minutes each after the peroxidase detection.
2. Add one drop of chromogenic reagent A and B respectively into 1 ml of distilled water and mixed. Add it onto the sample and colorate in dark for 10-30 min.
3. Wash with distilled water to stop the reaction. Counterstain with methyl blue for 3-5 min or nonalcoholic hematoxylin (Mayer’ s) if necessary.
4. Seal with water-soluble sealants or glycerol. Observe with microscope.
NOTE
DO NOT Counterstain with alcoholic hematoxylin.
