EASYspin RNA Isolation Kit (For yeast)
Size: 50 T
Lot # Check on the product label
--------------------------------------------------------------------------------------------------------------
Price: $250.00
Catalog# BPC1012
Size: 50 T
Lot # Check on the product label
Description
After yeast cells are treated with lyticase to remove the cell wall, the unique lysis buffer/ β-Mercaptoethanol immediately lyses biological samples and inactivates RNase and DNase. Ethanol is added to the lysate to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O.
Kit Contents and Storage
|
Components |
Size |
Storage |
|
Buffer SE |
30 ml |
Store at RT for 12 months. |
|
Lyticase 10 U/μl |
2500 U |
Store at -20°C for 12 months. |
|
Buffer RLT |
20 ml |
Store at RT for 12 months.
|
|
Buffer RW1 |
40 ml |
|
|
Wash Buffer RW |
10 ml |
|
|
RNase-free H2O |
10 ml |
|
|
RNA Bind Columns |
50 pcs |
