Taq DNA Polymerase (Buffer without Mgcl2)
Lot # Check on the product label
Size: 1000 U
Concentration: 5 U/µl
Storage and Expiration: Store at -20°C.
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Price: $90.00
Catalog# BPC1039-2
Lot # Check on the product label
Size: 1000 U
Concentration: 5 U/µl
Storage and Expiration: Store at -20°C.
Application: For PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, blunt-end addition of A, etc. The product can be directly used for T/A vector cloning.
Description: Taq DNA Polymerase is isolated and purified from E.coli cloned with Thermu aquaticus DNA Polymerase gene after induction table. Its molecular weight is 94 KD. Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity. In the PCR reaction, the extension speed of Taq DNA Polymerase is 1-2kb/min, and the product with A at the 3'end, which can be directly used for T/A vector cloning.
Activity definition: The activity of 1 unit (U) of Taq DNA Polymerase is defined as at 74°C for 30 min, use activated salmon sperm DNA as template primer, then add 10nmol of deoxynucleotides to the amount of enzyme required for acid-insoluble substances.
Enzyme storage buffer:
20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, 100 mM KCl, Stabilizers, 50% glycerol.
10×Taq Buffer (without Mg2+):
200 mM Tris-HCl (pH 8.4), 200 mM KCl, 100 mM(NH4)2 SO4, 15 mM MgCl2, and other components.
1. 10× Taq Buffer is divided into Mg2+ and without Mg2+, the user can choose.
2. Buffer without Mg2+, will be equipped with 25 mM MgCl2 additionally.
3. If not specified, it is usually provided that contains Mg2+ Buffer.
Kit Components
Components |
Size |
Taq DNA Polymerase |
1000 U |
10×Taq Buffer+ (with Mgcl2) |
2×1ml |
Recommended PCR conditions (Example: 50 µl)
Component |
Volume |
Template |
<0.5 μg |
Forward Primer (10 μM) |
1 μl |
Reverse Primer (10 μM) |
1 μl |
10×Buffer+ (with mgcl2) |
5 μl |
dNTP Mixture (2.5mM each) |
4 μl |
Taq DNA polymerase (5U/μl) |
0.5~1 μl |
dH2O |
up to 50 μl |