Taq DNA Polymerase (Buffer without Mgcl2)

Catalog# BPC1039-3

Lot # Check on the product label

Size: 3000 U

Concentration: 5 U/µl

Storage and Expiration: Store at -20°C.

Application: For PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, blunt-end addition of A, etc. The product can be directly used for T/A vector cloning.

Description: Taq DNA Polymerase is isolated and purified from E.coli cloned with Thermu aquaticus DNA Polymerase gene after induction table. Its molecular weight is 94 KD. Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity. In the PCR reaction, the extension speed of Taq DNA Polymerase is 1-2kb/min, and the product with A at the 3'end, which can be directly used for T/A vector cloning.

Activity definition: The activity of 1 unit (U) of Taq DNA Polymerase is defined as at 74°C for 30 min, use activated salmon sperm DNA as template primer, then add 10nmol of deoxynucleotides to the amount of enzyme required for acid-insoluble substances.

Enzyme storage buffer:

20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, 100 mM KCl, Stabilizers, 50% glycerol.

10×Taq Buffer (without Mg²⁺):

200 mM Tris-HCl (pH 8.4), 200 mM KCl, 100 mM(NH₄)₂ SO₄, 15 mM MgCl₂, and other components.

a. 10× Taq Buffer is divided into Mg²⁺ and without Mg²⁺, the user can choose.

b. Buffer without Mg²⁺, will be equipped with 25 mM MgCl₂ additionally.

c. If not specified, it is usually provided that contains Mg²⁺ Buffer.

Kit Components

Recommended PCR conditions (Example: 50 µl)