Polyclonal Anti- Collagen I/ COL1A1 antibody

Catalog# YPA2378

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat, bovine

Immunogen A synthesized peptide derived from human Collagen I, corresponding to a region within N-terminal amino acids.

Purification Purified by peptide affinity chromatography

Content Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Recommend Application

Western Blot, WB (1:250-1:500)

Immunohistochemistry, IHC (1:20-1:100)

Immunocytochemistry, ICC/IF (1:50-250) 

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.                    

Reference

1. Aihara, M., Lindsey, J. D., Weinreb, R. N. Ocular hypertension in mice with a targeted type I collagen mutation. Invest. Ophthal. Vis. Sci. 44: 1581-1585, 2003.

2. Cabral, W. A., Makareeva, E., Colige, A., Letocha, A. D., Ty, J. M., Yeowell, H. N., Pals, G., Leikin, S., Marini, J. C. Mutations near amino end of alpha-1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J. Biol. Chem. 280: 19259-19269, 2005.