Polyclonal Anti- PPAR gamma antibody

Catalog#  YPA2204

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPAR gamma.

Purification Purified by immunogen affinity chromatography

Content Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Recommend Application

Western Blot, WB (1:500-1:1000)

Immunohistochemistry, IHC (1:100-1:200)

Immunocytochemistry, ICC/IF (1:100-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Peroxisome proliferator-activated receptor gamma (PPAR-γ or PPARG), also known as the glitazone reverse insulin resistance receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3) is a type II nuclear receptor functioning as a transcription factor that in humans is encoded by the PPARG gene.

Reference

1. Greene ME, Blumberg B, McBride OW, Yi HF, Kronquist K, Kwan K, et al. (1995). "Isolation of the human peroxisome proliferator activated receptor gamma cDNA: expression in hematopoietic cells and chromosomal mapping". Gene Expression. 4 (4–5): 281–99.

2. Elbrecht A, Chen Y, Cullinan CA, Hayes N, Leibowitz MD, Moller DE, Berger J (July 1996). "Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors gamma 1 and gamma 2". Biochemical and Biophysical Research Communications. 224 (2): 431–7.

3. Michalik L, Auwerx J, Berger JP, Chatterjee VK, Glass CK, Gonzalez FJ, et al. (December 2006). "International Union of Pharmacology. LXI. Peroxisome proliferator-activated receptors". Pharmacological Reviews. 58 (4): 726–41.