pZERO-Blunt zero background Blunt Quick Ligation Kit
Lot # Check on the product label
Size: 20 T
Storage: Store at -20°C for 6 months.
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Price: $190.00
Catalog# BPC1091-1
Lot # Check on the product label
Size: 20 T
Storage: Store at -20°C for 6 months.
Description
The Zero Background Blunt-End Quick Ligation Kit is an advanced suicide gene positive selection cloning system that efficiently clones blunt-ended PCR products and any DNA fragments with blunt ends. The kit is effective on both phosphorylated and non-phosphorylated DNA fragments. The positive selection vector and insert ligation can obtain more than 90% of positive recombinant clones in only 5 minutes. Blunt-ended PCR products amplified by a proofreading polymerase (eg, Pfu polymerase) can be ligated directly into cloning vectors. The ligation product can be directly transformed into commonly used E. coli strains.
Components
|
Components |
Size |
|
pZERO-Blunt Simple Vector(40 ng/μl) |
20 µl |
|
900bp Control(40ng/µl) |
5 µl |
|
2x Quick Ligation Buffer |
100 µl |
|
T4 DNAligase, 5U/μl |
20 µl |
|
pZERO-Blunt Simple Forward Sequencing Primer (10µM) |
50 µl |
|
pZERO/Blunt Simple Reverse Sequencing Primer (10µM) |
50 µl |
