Monoclonal Anti-Myc Tag (HRP Conjugated)

Catalog# BTL1017

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Specificity

Detect over-expressed or recombinant proteins containing the Myc epitope tag.

Product Form Liquid (1mg/ml)

Immunogen 

A synthetic peptide (conjugated to KLH) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Recommend Application

Western Blot (1:5000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.

Avoid repeated freeze and thaw cycles.

Background

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, over expressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein.