EASYspin Plus RNA Extraction Kit (For Tissues & Cells)

Catalog# BPC1015

Lot # Check on the product label

Size: 50 Tests

Storage and Expiration: When store in indicated temperature, are stable for 9 months.

Description

The innovative EASYspin plus system is ideal or rapid purification of genomic DNA-free total RNA from animal cells or tissues. The unique genomic DNA elimination columns effectively remove genomic DNA from RNA samples. No DNase digestion step is required. The purified total RNA can be directly used for reverse transcription and RT-PCR. The whole process is phenol/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase. The lysate containing RNA is then passed through a genomic DNA elimination column, where genomic DNA binds to the column membrane, and RNA remains in the flow-through. Ethanol is added to the flow-through to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica-membrane with RNase-free H₂O.

Kit Contents and Storage

Storage precautions

1. All reagent solutions should be clear. If the ambient temperature is low, the solution may precipitate and should not be used directly. It can be heated in a 37°C water bath and used after it is clear.