EASYspin Plus RNA IsolationKit (For bulk Tissues & Cells)
Lot # Check on the product label
Size: 10 T
Storage and Expiration: When store in indicated temperature, are stable for 9 months.
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Price: $700.00
Catalog# BPC1016
Lot # Check on the product label
Size: 10 T
Storage and Expiration: When store in indicated temperature, are stable for 9 months.
Application
It is used for rapid extraction of total RNA from animal cells and easily lysed animal tissues. The unique genomic DNA removal column technology is used to ensure the effective removal of gDNA residues. DNase digestion is not required. RNA can be directly used for PCR and fluorescent quantitative PCR.
Description
The innovative EASYspin plus system is ideal or rapid purification of genomic DNA-free total RNA from animal cells or tissues. The unique genomic DNA elimination columns effectively remove genomic DNA from RNA samples. No DNase digestion step is required. The purified total RNA can be directly used for reverse transcription and RT-PCR. The whole process is phenol/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase. The lysate containing RNA is then passed through a genomic DNA elimination column, where genomic DNA binds to the column membrane, and RNA remains in the flow-through. Ethanol is added to the flow-through to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica-membrane with RNase-free H2O.
Kit Contents and Storage
Components |
Size |
Storage |
Buffer RLT Plus |
100 ml |
Store at RT for 6 months.
|
Buffer RW1 |
120 ml |
|
Wash Buffer RW |
25 ml x2 |
|
70% Ethanol |
15ml x2 |
|
RNase-free H2O |
10 ml |
|
Genomic DNA Elimination Columns |
10 pcs |
|
RNA Binding Columns |
10 pcs |
Storage precautions
1. All reagent solutions should be clear. If the ambient temperature is low, the solution may precipitate and should not be used directly. It can be heated in a 37°C water bath and used after it is clear.