Monoclonal Anti- Aflatoxins B1+B2+G1+G2 Antibody

Catalog# BDA1021

Lot # Check on the product label

Size 1 mg

Host Mouse

Product Form Liquid

Concentration 1.8 mg/ml (Changed for different lots)

Purification & Buffer

Protein A or G purified and supplied in 0.01 M PBS (pH7.4)

Purity >95% by HPLC & SDS-PAGE

IC50 Value

0.05-0.1 ppb

Sensitivity 0.01 ppb for ELISA, 0.5 ppb for Lateral Flow Assay

Specificity Has 100%, 56%, 92% & 77% cross-reactivities with Aflatoxins B1, Aflatoxins B2, Aflatoxins G1 & Aflatoxins G2 respectively.

Recommend Application

ELISA (1:100000)

Lateral Flow Assay

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Aliquot and store at -20°C for 2 years.

Avoid repeated freeze and thaw cycles.

Background

Aflatoxins are naturally occurring mycotoxins that are produced by Aspergillus flavus and Aspergillus parasiticus, species of fungi. Aspergillus, Penicillium, and Fusarium species frequently contaminate crops. For this reason mycotoxins such as aflatoxins (AFs), ochratoxin A (OTA), fumonisins (FBs), and zearalenone (ZEA) are found in food and feed in a wide range of concentrations, depending on environmental and storage conditions. Consumption of mycotoxin-contaminated food and feed has been associated with acute and chronic poisoning and carcinoma.  Co-occurrence of two toxins varied between 4.2% and 54% and of three between 4.2% and 7.6%. Prolonged co-exposure to AFs, OTA, FBs, and ZEA might increase the risk of various chronic diseases. The stress-induced compound, 6,7-dimethoxycoumarin, isolated from P. digitatum-infected Valencia fruit confers resistance against the mycotoxigenic fungi A. parasiticus and F. verticillioides and causes a reduction in production of fumonisin B1 and aflatoxins G1, G2, B1, and B2.

Reference

1. Klarić MS, Cvetnić Z, Pepeljnjak S, Kosalec I. Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography. Arh Hig Rada Toksikol. 2009 Dec;60(4):427-34.

2. Mohanlall V, Odhav B. Biocontrol of aflatoxins B1, B2, G1, G2, and fumonisin B1 with 6,7-dimethoxycoumarin, a phytoalexin from Citrus sinensis. J Food Prot. 2006 Sep;69(9):2224-9.