Catalog# BPC1086-2
Lot # Check on the product label
Size: 80T×10 μl
Storage and Expiration: Store at -20°C for one year.
Description:
The Zero Background pTOPO-Blunt Simple Cloning Kit utilizes the principle of Topoisomerase to connect DNA fragments in an instant (a few seconds to a few minutes) ,it efficiently connect any PCR product (compatible with A-end/blunt-end). This kit does not contain polyclonal restriction sites on both sides of the cloning insertion site. It is necessary to introduce appropriate restriction sites on the PCR amplification primers.
Note: Sequencing can be sequenced with M13F/M13R universal primers (see map at end)
Kit Components
Components |
Size |
pTOPO-TA/Blunt Simple Vector(30 ng/μl) |
80 μl |
1kb Control(40 ng/μl) |
5 μl |
10×Enhancer |
80 μl |
Details